The zinc finger protein A20 protects endothelial cells from burns serum injury

Zhu, Chu-hong; Ying, Da-jun; Mi, Jian-hong; Zhang, Wei; Dong, Shi-wu; Sun, Jian-sen; Zhang, Jia-ping

doi:10.1016/j.burns.2003.08.010

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Volume 30, Issue 2 , Pages 127-133, March 2004

The zinc finger protein A20 protects endothelial cells from burns serum injury

Chu-hong Zhu
- Department of Anatomy, The Key Lab of Biomechanics under the Ministry, The Third Military Medical University, Gao Tan Yan Street, Shaping Ba District, Chongqing 400038, China
Da-jun Ying
- Department of Anatomy, The Key Lab of Biomechanics under the Ministry, The Third Military Medical University, Gao Tan Yan Street, Shaping Ba District, Chongqing 400038, China
- Corresponding author. Tel.: +86-236-8752225; fax: +86-236-8752226.
Jian-hong Mi
- Department of Anatomy, The Key Lab of Biomechanics under the Ministry, The Third Military Medical University, Gao Tan Yan Street, Shaping Ba District, Chongqing 400038, China
Wei Zhang
- Department of Anatomy, The Key Lab of Biomechanics under the Ministry, The Third Military Medical University, Gao Tan Yan Street, Shaping Ba District, Chongqing 400038, China
Shi-wu Dong
- Department of Anatomy, The Key Lab of Biomechanics under the Ministry, The Third Military Medical University, Gao Tan Yan Street, Shaping Ba District, Chongqing 400038, China
Jian-sen Sun
- Department of Anatomy, The Key Lab of Biomechanics under the Ministry, The Third Military Medical University, Gao Tan Yan Street, Shaping Ba District, Chongqing 400038, China
Jia-ping Zhang
- Institute of Burns Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China

Accepted 12 August 2003.

Abstract

Burn injuries as well as skin damages are often associated with immune suppression and often cause multiple organ failures. The monolayer endothelium is vulnerable to injuries from circulating factors resulting from remote wounds. Endothelial cell activation and apoptosis can alter microvascular permeability and intensify organ damage. A20, as a physiological cytoprotective gene is essential for preventing spontaneous innate immune cell-mediated inflammation and tissue destruction. It is not known whether A20 has the function to protect endothelial cells from the effect of burns serum challenge on endothelial function in vitro. This study shows that A20 can express in endothelial cells after burns serum stimulation and inhibit endothelial cell activation and apoptosis induced by burns serum. These results suggest that A20 may be beneficial in limiting the response to burn injuries.

Keywords: A20, Endothelial cells (ECs), Burn injuries, Apoptosis, NF-kappaB, Inflammation, Activation