Burns
Volume 31, Issue 1 , Pages 25-29 , February 2005

The Viennese culture method: cultured human epithelium obtained on a dermal matrix based on fibroblast containing fibrin glue gels

  • L.P. Kamolz

      Affiliations

    • Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
    • Corresponding Author InformationCorresponding author. Tel.: +431 40400 6986; fax: +431 4040 6988.
    • ,
  • M. Luegmair

      Affiliations

    • Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
    • ,
  • N. Wick

      Affiliations

    • Institute of Pathology, Medical University of Vienna, Vienna, Austria
    • ,
  • B. Eisenbock

      Affiliations

    • Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
    • ,
  • S. Burjak

      Affiliations

    • Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
    • ,
  • R. Koller

      Affiliations

    • Department of Plastic and Reconstructive Surgery, Wilhelminen Hospital, Vienna, Austria
    • ,
  • G. Meissl

      Affiliations

    • Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria
    • ,
  • M. Frey

      Affiliations

    • Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria

,Accepted 2 June 2004.

References 

  1. Rheinwald J, Green H. Serial cultivation of strain of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975;6:331–344
  2. De Luca M, Albanese E, Bondanza S, Megna M, Ugozzoli L, Molina F, et al. Multicentre experience in the treatmment of burns with autologus and allogenic cultured epithelim, fresh or preserved in a frozen state. Burns. 1989;15:303–309
  3. Teepe RG, Koebruge E, Ponec M, Vermeer BJ. Fresh versus cryopreserved cultured allografts for the treatment of chronic skin ulcers. Br. J. Dermatol. 1990;122:81–89
  4. De Luca M, Albanese E, Megna M, Cancedda R, Mangiante PE, Cadoni A, et al. Evidence that human oral epithelium reconstituted in vitro and transplanted onto patients with defects in the oral mucosa retains properties of the original donor site. Transplantation. 1990;50:454–459
  5. Tenchini ML, Ranzati C, Malcovati M. Culture techniques for human keratinocytes. Burns. 1992;S1:12–16
  6. Currie JL, Martin R, Sharpe JD, James SE. A comparison of keratinocyte cell sprays with and without fibrin glue. Burns. 2003;29:677–685
  7. Harris PA, Leigh IM, Navsaria HA. Pre-confluent keratinocyte grafting: the future for cultured skin replacements. Burns. 1998;24:591–593
  8. Meana A, Iglesias J, Del Rio M, Larcher F, Madrigal B, Fresno MF, et al. Large surface of cultured human epithelium obtained on a dermal matrix based on live fibrobalst-containing fibrin gels. Burns. 1998;24:621–630
  9. Maraguchi T, Maraguchi Y, Suzuki S, Matsuda K, Toda K, Isshiki N. A new skin equivalent: keratinocytes proliferated and differentiated on collagen sponge containing fibroblast. Plast. Reconstr. Surg. 1994;93:537–544
  10. Boyce S, Hansbrough J. Biologic attachment, grow, and differentiation of cultured human epidermal keratinocytes on a graftable collagen and chondroitin-6-sulfate substrate. Surgery. 1988;103:421–431
  11. Saintigny G, Bonnard M, Damour O, Collombel C. Reconstruction of epidermis on a chitosan cross-linked collagen-GAG lattice: effect of fibroblast. Acta Dermatol. Venereol. 1993;73:175–180
  12. Myers S, Navsaria H, Sanders R, Green C, Leigh I. Transplantation of keratinocytes in the treatment of wounds. Am. J. Surg. 1995;170:75–83
  13. Nanchahal J, Ward CM. New grafts for old? A review of alternatives to autologous skin. Br. J. Plastic Surg. 1992;45:354–363
  14. Deblois C, Côté M, Doillon C. Heparin–fibroblast growth factor-fibrin complex: in vitro and in vivo applications to collagen-based materials. Biomaterials. 1994;15:665–672
  15. Brown LF, Lanir N, Mcdonagh J, Tognazzi K, Dvorak Am, Dvorak HF. Fibroblast migration in fibrin gel matrices. Am. J. Pathol. 1993;142:273–283
  16. Ronfard V, Rives JM, Neveux Y, Carsin N, Barrandon Y. Long term regeneration of human epidermis on third degree burns transplanted with autologous cultured epithelium grown on a fibrin matrix. Transplantation. 2000;70:1588–1598
  17. Kaiser HW, Stark GB, Kopp J, Balcerkiewicz A, Spilker G, Kreysel HW. Cultured autologous keratinocytes in fibrin glue suspension, exclusively and combined with STS-allograft (preliminary clinical and histological report of a new technique). Burns. 1994;20:23–29
  18. Stark GB, Kaiser HW. Cologne Burns Centre experience with glycerol-preserved allogenic skin. Part II. Combination with autologus cultured keratinocytes. Burns. 1994;20(Suppl. 1):34–38
  19. Currie LJ, Sharpe JR, Martin R. The use of fibrin glue in skin grafts and tissue engineered skin replacements: a review. Plast. Reconstr. Surg. 2001;108:1713–1726
  20. Ronfard V, Broly H, Mitchell V, Galizia JP, Hochart D, Chambon E, et al. Use of human keratinocytes cultured on fibrin glue in the treatment of burn wounds. Burns. 1991;17:181–184
  21. Martin P. Wound healing: aiming for perfect skin regeneration. Science. 1997;276:75–81

PII: S0305-4179(04)00185-8

doi: 10.1016/j.burns.2004.06.015

Burns
Volume 31, Issue 1 , Pages 25-29 , February 2005

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