Burns
Volume 31, Issue 6 , Pages 707-710 , September 2005

Prevalence of metallo-β-lactamase among Pseudomonas aeruginosa and Acinetobacter baumannii isolated from burn wounds and in vitro activities of antibiotic combinations against these isolates

  • Ulku Altoparlak

      Affiliations

    • Department of Microbiology and Clinical Microbiology, Ataturk University, Tip Fakultesi, Aziziye Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, School of Medicine, Erzurum 25100, Turkey
    • Corresponding Author InformationCorresponding author. Tel.: +90 442 3166333/1187.
    • ,
  • Ferda Aktas

      Affiliations

    • Department of Microbiology and Clinical Microbiology, Ataturk University, Tip Fakultesi, Aziziye Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, School of Medicine, Erzurum 25100, Turkey
    • ,
  • Demet Celebi

      Affiliations

    • Department of Microbiology and Clinical Microbiology, Ataturk University, Tip Fakultesi, Aziziye Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, School of Medicine, Erzurum 25100, Turkey
    • ,
  • Zulal Ozkurt

      Affiliations

    • Department of Clinical Bacteriology and Infectious Diseases, Ataturk University, School of Medicine, Erzurum, Turkey
    • ,
  • Mufide N. Akcay

      Affiliations

    • Department of General Surgery, Burn Unit, Ataturk University, School of Medicine, Erzurum, Turkey

,Accepted 10 February 2005.

References 

  1. Agnihotri N, Gupta V, Joshi RM. Aerobic bacterial isolates from burn wound infections and their antibiograms-a five-year study. Burns. 2004;30:241–243
  2. Santucci SG, Gobara S, Santos CR, Fontana C, Levin AS. Infections in a burn intensive care unit: experience of seven years. J Hosp Infect. 2003;53:6–13
  3. Rhomberg PR, Jones RN, Sader HS. MYSTIC Programme (US) Study Group. Results from the Meropenem Yearly Susceptibility Test Information Collection (MYSTIC) Programme: report of the 2001 data from 15 United States Medical Centres. Int J Antimicrob Agents. 2004;23:52–59
  4. Yan JJ, Wu JJ, Tsai SH, Chuang CL. Comparison of the double-disk, combined disk, and E-test methods for detecting metallo-β-lactamases in Gram-negative bacilli. Diagn Microbiol Infect Dis. 2004;49:5–11
  5. Arakawa Y, Shibata N, Shibayama K, Kurokawa H, Yagi T, Fujiwara H, et al. Convenient test for screening metallo-β-lactamase-producing Gram-negative bacteria by using thiol compounds. J Clin Microbiol. 2000;38:40–43
  6. National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility Tests, 6th ed. Approved Standard. NCCLS Document M2-A6, vol. 17, No. 1. Pennsylvania.
  7. Mayer I, Nagy E. Investigation of the synergic effects of aminoglycoside-fluoroquinolone and third-generation cephalosporin combinations against clinical isolates of Pseudomonas spp. Antimicrob Chemother. 1999;43:651–657
  8. Lorian V. Antibiotics in laboratory medicine. 4th ed.. Baltimore, MD: Williams and Wilkins; 1996;
  9. EUCAST. Methods for the determination of susceptibility of bacteria to antimicrobial agents. Terminology, EUCAST Document, January, 1998.
  10. Yong D, Lee K, Yum JH, Shin HB, Rossolini GM, Chong Y. Imipenem-EDTA disk method for differentiation of metallo-β-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol. 2002;40:3798–3801
  11. Luzzaro F, Endimiani A, Docquier JD, Mugnaioli C, Bonsignori M, Amicosante G, et al. Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa. Diagn Microbiol Infect Dis. 2004;48:131–135
  12. Takahashi A, Yomoda S, Kobayashi I, Okubo T, Tsunoda M, Iyobe S. Detection of cabapenemase-producing Acinetobacter baumannii in a hospital. J Clin Microbiol. 2000;38:526–529
  13. Oh EJ, Lee S, Park YJ, Park JJ, Park K, Kim SI, et al. Prevalence of metallo-β-lactamase among Pseudomonas aeruginosa and Acinetobacter baumannii in a Korean university hospital and comparison of screening methods for detecting metallo-β-lactamase. J Microbiol Methods. 2003;54:411–418
  14. Lee K, Lim JB, Yum JH, Yong D, Chong Y, Kim JM, et al. bla(VIM-2) cassette-containing novel integrons in metallo-β-lactamase-producing Pseudomonas aeruginosa and Pseudomonas putida isolates disseminated in a Korean hospital. Antimicrob Agents Chemother. 2002;46:1053–1058
  15. Lee K, Lee WG, Uh Y, Ha GY, Cho J, Chong Y. Korean Nationwide Surveillance of Antimicrobial Resistance Group. VIM- and IMP-type metallo-β-lactamase-producing Pseudomonas spp. and Acinetobacter spp. in Korean hospitals. Emerg Infect Dis. 2003;9:868–871
  16. Lauretti L, Riccio ML, Mazzariol A, Cornaglia G, Amicosante G, Fontana R, et al. Cloning and characterization of blaVIM, a new integron-borne metallo-β-lactamase gene from a Pseudomonas aeruginosa clinical isolate. Antimicrob Agents Chemother. 1999;43:1584–1590

PII: S0305-4179(05)00086-0

doi: 10.1016/j.burns.2005.02.017

Burns
Volume 31, Issue 6 , Pages 707-710 , September 2005

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