Sepsis and burn complicated by sepsis alter cardiac transporter expression

Intracellular calcium concentration in cardiomyocytes following 24h sepsis, 48h burn (Day2) complicated by 24h sepsis or 72h burn (Day 3) plus 24h sepsis. Myocytes were isolated from 5–6 rats, loaded with Fura 2AM, and fluorescence measured. Approximately 20 cardiomyocytes from each rat were included for study. All values are mean±S.E.M. Asterisk (*) indicates significant difference from control at p<0.05.

(A). Western blot analysis showing increased and decreased SERCA expression at various times after induction of sepsis. Cont=sham. (B). Densitometric analysis of several western blots (N=3–4) at each time period. All values are mean±S.E.M. Asterisk(*) indicates significant difference from control at p<0.05.

(A). Representative blot depicting the time course of SERCA expression in 48h burns complicated by sepsis. Cont=sham. (B). Densitometric summary of data (N=8). (C). SERCA protein levels in 72h burns complicated by sepsis as measured by western blot analysis. (D). Densitometric summary of data (N=4). All values are mean±S.E.M. Asterisk(*) indicate significant difference from control at p<0.05.

(A). Effects of sepsis on myocardial L-type Ca²⁺ channel expression as determined by western blot analysis and (B). densitometric analysis of several westerns (N=4–6) at each time post sepsis. All values are mean±S.E.M. Asterisk(*) indicate significant difference from control at p<0.05.

(A). L-type Ca²⁺ channel expression in 48h burns plus sepsis groups and (B). the densitometric analysis (N=8). (C). The protein levels of L-type Ca²⁺ channel following 72h burns complicated by various times of sepsis. (D). Densitometric summary of data. All values are mean±S.E.M. Asterisk(*) indicates significant difference from control at p<0.05.

(A). Sepsis-related changes in myocardial Na⁺/Ca²⁺ exchanger protein as determined by western blot analysis and (B). densitometric summary of several (N=3–5) westerns at each time point. All values are mean±S.E.M. Asterisk(*) indicates significant difference from control at p<0.05.

(A). Expression of the Na⁺/Ca²⁺ exchanger in 48h burns complicated by sepsis as measured by western blot analysis and (B). the densitometric summary of the western data (N=3). (C&D). Exchanger protein levels following 72h burns plus sepsis. All values are mean±S.E.M. Asterisk(*) indicates significant difference from control at p<0.05.

Time course of sodium accumulation by cardiomyocytes after sepsis or burn complicated by sepsis. Myocytes were prepared from 5–6 rats at each time point. At least 20 myocytes from each rat were included for each time point. All values are mean±S.E.M. Asterisk(*) indicates significant difference from control at p<0.05.

(A). Effects of sepsis on myocardial Na⁺/K⁺ ATPase protein expression as measured by western blot analysis and (B). densitometry. All values are mean±S.E.M. Asterisk(*) indicates significant difference from control at p<0.05.

Western blot analysis and densitometric summary depicting cardiac Na⁺/K⁺ ATPase expression in 48h burns complicated by sepsis (A&B) or in 72h burns plus sepsis (C&D). All values are mean±S.E.M. Asterisk(*) indicates significant difference from control at p<0.05.