Burns
Volume 34, Issue 8 , Pages 1128-1136 , December 2008

Cellular recovery from electroporation using synchronisation modulation as a rescue model for electrically injured cells

  • Robin Dando
    • ,
  • Wei Chen

      Affiliations

    • Corresponding Author InformationCorresponding author at: Laboratory for Cellular and Molecular Biophysics, Department of Physics, University of South Florida, 4202 E. Fowler Avenue, PHY 114, Tampa, FL 33620, United States. Tel.: +1 813 974 5038.

,Accepted 21 January 2008.

References 

  1. Chen W. Synchronization of carrier-mediated pump molecules by an oscillating electric field: theory. J Phys Chem; submitted for publication.
  2. Chen W, Huang F. Computer simulation of electrical synchronization of the Na/K pumps; J. Bioenerg. Biomem., submitted for publication.
  3. Chen W, Zhang ZS. Synchronization of Na/K pump molecules by a train of squared pulses. J Bioenerg Biomembr. 2006;38:319–325
  4. Chen W, Zhang ZS, Huang F. entrainment of the Na/K pump molecules by an oscillating electric field. J. Bioenerg. Biomembr. 2007;39:331–339
  5. Chen W, Dando R. Synchronization modulation of Na/K pump molecules can hyperpolarize the membrane resting potential in intact fibers. J Bioenerg Biomembr. 2007;39:117–126
  6. Chen W, Dando R. Electrical activation of Na/K pumps can increase ionic concentration gradient and membrane resting potential. J Membr Biol. 2006;39:117–126
  7. Chen W, Dando R. Synchronization modulation of Na/K pumps can hyperpolarize membrane potential in mammalian cardiac cells. J Membr Biol; in press.
  8. Chen W, Wang L. Synchronization modulation of the Na/K pump molecules can hyperpolarize the membrane potential of PC12 cells. Mol Cell Biomech. 2006;4:203–204
  9. In:  Neumann E,  Sowers A,  Jordan C editor. Electroporation and electrofusion in cell biology. New York: Plenum; 1989;
  10. Lee RC, Gaylor DG, Bhatt DL, Israel DA. Role of cell membrane rupture in the pathogenesis of electrical trauma. J Surg Res. 1988;44:709
  11. Tsong TY. Electroporation of cell membranes. Biophys J. 1991;60:297–306
  12. Weaver JC. Electroporation: a general phenomenon for manipulating cells and tissues. J Cell Biochem. 1993;51:426–435
  13. Chen W, Lee RC. An improved double Vaseline gap voltage clamp to study electroporated skeletal muscle fibers. Biophys J. 1994;66:700–709
  14. Chen W, Lee RC. Altered channel conductance and selectivity induced by imposed membrane potential pulses. Biophys J. 1994;67:603–612
  15. Chen W, Han Y, Chen Y, Astumian D. Electric field-induced functional reductions in the K+ channels mainly resulted from supramembrane potential-mediated electroconformational changes. Biophys J. 1998;75:196–206
  16. Lee RC, Dougherty W. Electrical injury: mechanisms, manifestations, and therapy. IEEE Trans Dielectr Electr Insul. 2003;10:810–819
  17. Bier M, Chen W, Bodnar E, Lee R. Biophysical injury mechanisms associated with lightning injury. NeuroRehabilitation. 2005;20
  18. Hille B, Campbell DT. An improved Vaseline-gap voltage-clamp for skeletal muscle fibers. J Gen Physiol. 1976;67:265–293
  19. Kovacs L, Schumperli RA, Szucs G. Comparison of birefringence signals and calcium transients in voltage-clamped cut skeletal muscle fibers of the frog. J Physiol. 1983;341:579–593
  20. Irving M, Maylie J, Sizto NL, Chandler WK. Intrinsic optical and passive electrical properties of cut frog twitch fibers. J Gen Physiol. 1987;89:1–40
  21. Hui CS, Chen W. Separation of Q beta and Q gamma charge components in frog cut twitch fibers with tetracaine. Critical comparison with other methods. J Gen Physiol. 1992;99:985–1016
  22. O’Neill RJ, Tung L. Cell-attached patch clamp study of the electropermeabilization of amphibian cardiac cells. Biophys J. 1991;59:1028–1039
  23. Waggoner AS. Optical probes of membrane potential. J Membr Biol. 1976;27:317–334
  24. Loew LM. Confocal microscopy of potentiometric fluorescent dyes. Methods Cell Biol. 1993;8:195–209
  25. Cole KS. Membrane, ions and impulses. Berkeley, CA: University of California Press; 1972;p. 569–90
  26. Lee RC, River LP, Pan FS, Li J, Wollmann RL. Surfactant-induced sealing of electropermeabilized skeletal muscle membrane in vivo. Proc Natl Acad Sci USA. 1992;89:4524–4528
  27. Neunlist M, Tung L. Dose-dependent reduction of cardiac transmembrane potential by high-intensity electrical shocks. Am J Physiol. 1997;273:H2817–H2825
  28. Clausen T. Na/K pump regulation and skeletal muscle contractility. Physiol Rev. 2003;83:1269–1324

PII: S0305-4179(08)00040-5

doi: 10.1016/j.burns.2008.01.020

Burns
Volume 34, Issue 8 , Pages 1128-1136 , December 2008

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