Burns
Volume 35, Issue 4 , Pages 527-537, June 2009

Recombinant human decorin inhibits TGF-β1-induced contraction of collagen lattice by hypertrophic scar fibroblasts

  • Zhi Zhang

      Affiliations

    • Department of Burn and Plastic Surgery, Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China
    • Corresponding Author InformationCorresponding author at: Department of Burn and Plastic Surgery, Guangzhou Red Cross Hospital, No. 396 Tong Fu Zhong Road, Hai Zhu District, Guangzhou, Guangdong Province 510220, China. Tel.: +86 20 3437 1669.
    • ,
  • Tania M. Garron

      Affiliations

    • Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA
    • ,
  • Xiao-Jian Li

      Affiliations

    • Department of Burn and Plastic Surgery, Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China
    • ,
  • Yan Liu

      Affiliations

    • Shanghai Institute of Burn, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai, China
    • ,
  • Xiong Zhang

      Affiliations

    • Shanghai Institute of Burn, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai, China
    • ,
  • Ye-Yang Li

      Affiliations

    • Department of Burn and Plastic Surgery, Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China
    • ,
  • Wei-Shi Xu

      Affiliations

    • Shanghai Institute of Burn, Ruijin Hospital, Shanghai Jiao Tong University, Shanghai, China

Accepted 25 August 2008.

Abstract 

Decorin was reported to bind transforming growth factor-beta (TGF-β1) and neutralise some of its activity as a key regulator of wound contraction and hypertrophic scar formation. In this study, we investigated whether recombinant human decorin affected TGF-β1-induced fibroblast contractile activity, by using fibroblast-populated collagen lattice with decorin added to the collagen gel. Hypertrophic scar fibroblasts showed greater basal contraction of collagen gels than normal fibroblasts, and the addition of TGF-β1 significantly enhanced this. Decorin inhibited both the basal and TGF-β1-enhanced contraction of collagen gel by both normal and hypertrophic scar fibroblasts. Decorin also inhibited TGF-β1-induced α-smooth muscle actin (α-SMA), plasminogen activator inhibitor-1 (PAI-1) protein and mRNA expressions in normal and hypertrophic scar fibroblasts. These results suggest that decorin may have therapeutic potential for excessive skin contraction as observed in hypertrophic scarring.

Keywords: Decorin, TGF-β1, Fibroblasts, Hypertrophic scar, Contraction

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PII: S0305-4179(08)00280-5

doi:10.1016/j.burns.2008.08.021

Burns
Volume 35, Issue 4 , Pages 527-537, June 2009

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