Burns
Volume 36, Issue 3 , Pages 347-351 , May 2010

Processing efficacy in relation to microbial contamination of skin allografts from 723 donors

  • E. Pianigiani

      Affiliations

    • Siena Skin Bank, Dept. Dermatology, Policlinico Santa Maria alle Scotte, Viale Bracci, 53100 Siena, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39 0577 585425; fax: +39 0577 585327.
    • ,
  • F. Ierardi

      Affiliations

    • Siena Skin Bank, Dept. Dermatology, Policlinico Santa Maria alle Scotte, Viale Bracci, 53100 Siena, Italy
    • ,
  • C. Cuciti

      Affiliations

    • Siena Skin Bank, Dept. Dermatology, Policlinico Santa Maria alle Scotte, Viale Bracci, 53100 Siena, Italy
    • ,
  • S. Brignali

      Affiliations

    • Dept. Microbiology, University of Siena, Siena, Italy
    • ,
  • M. Oggioni

      Affiliations

    • Dept. Microbiology, University of Siena, Siena, Italy
    • ,
  • M. Fimiani

      Affiliations

    • Siena Skin Bank, Dept. Dermatology, Policlinico Santa Maria alle Scotte, Viale Bracci, 53100 Siena, Italy

,Accepted 17 April 2009.

References 

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  2. Girdner JH. Skin grafting with grafts taken from the dead subject. Med. Rec. 1881;20:119–120
  3. Alexander JW, McMillan BG, Law E, Kittur DS. Treatment of severe burns with widely meshed skin autograft and meshed skin allograft overlay. J. Trauma. 1981;21:433–438
  4. Hermans MHE. Clinical experience with glycerol-preserved donor skin treatment in partial thickness burns. Burns. 1989;15:57–59
  5. Horch R, Stark GB, Kopp J, Spiker G. Cologne Burn Center experiences with glycerol-preserved allogeneic skin: clinical experiences and histological findings (overgraft and sandwich technique). Burns. 1994;20:S23–S26
  6. Heck EL, Bergstrasser PR, Baxter C. Composite skin graft: frozen dermal allografts support the engraftment and expansion of autologous epidermis. J. Trauma. 1985;25:106–112
  7. Cuono CB, Langdon R, Birchall N, Barttelbort S, McGuire J. Composite autologous-allogeneic skin replacement: development and clinical application. Plast. Recontr. Surg. 1987;80:626–635
  8. Witt PD, Cohen DT, Mallory SB. Use of a permanent acellular dermal allograft in recessive dystrophic epidermolysis bullosa involving the hands. Arch. Dermatol. 1999;135:503–506
  9. Horch RE, Jeschkea MG, Spilkerb G, Herndonc DN, Koppa J. Treatment of second degree facial burns with allografts-preliminary results. Burns. 2005;31:597–602
  10. Pianigiani E, Ierardi F, Cherubini Di Simplicio F, Andreassi A. Skin bank organization. Clin. Dermatol. 2005;23:353–356
  11. Directive 2004/23/EC of the European Parliament and Council on quality and safety standards for the donation, procurement, testing, processing, preservation, storage and distribution of human tissues and cells.
  12. UNI EN ISO 9001:2000. Quality management system requirements, 31/12/2000.
  13. EC Guide to manufacturing practice, revision to annex 1, 2008.
  14. VanBaare J, Cameron PU, Vardaxis N, Pagnon J, Reece J, Middelkoop E, et al. Comparison of glycerol preservation with cryopreservation methods on HIV-1 inactivation. J. Burn Care Rehabil. 1998;19:494–500
  15. Blondet R, Gibert-Thevenin MA, Pierre C, Ehrsam A. Skin preservation by programmed freezing. Br. J. Plast. Surg. 1982;35:530–536
  16. May SR, Wainwringht JF, DeClement FA. Variables determinig the amount of microbial contamination on cadaveric allograft skin used as a biological wound dressing. Burns. 1985;11:242–251
  17. Van Baare J, Ligtvoet EEJ, Middelkoop E. Microbiological evaluation of glycerolized cadaveric donor skin. Transplantation. 1998;65:966–970
  18. Csönge L, Pellet S, Szenes A, Istvan J. Antibiotics in the preservation of allograft and xenograft skin. Burns. 1995;21:102–105
  19. Obeng MK, McCauley RL, Barnett JR, Heggers JP, Sheridan K, Schutzler SS. Cadaveric allograft discards as a result of positive skin cultures. Burns. 2001;27:267–271
  20. White MJ, Whalen JD, Gould JA, Brown GL, Polk HC. Procurement and transplantation of colonized cadaver skin. Am. Surg. 1991;6:402–407
  21. Bojar AR, Holland KT. Acne and Propionibacterium acnes. Clin. Dermatol. 2004;22:375–379
  22. Deijkers RL, Bloem RM, Petit IL, Brand R, Vehmeyer SB, Veen MR. Contamination of bone allografts: analysis of incidence and predisposing factors. J. Bone Joint Surg. Br. 1997;79:161–166
  23. Forsell JH, Liesman J. Analysis of potential causes of positive microbiological cultures in tissue donors. Cell Tissue Banking. 2000;1:111–115
  24. Mathur M, De A, Gore M. Microbiological assessment of cadaver skin grafts received in a skin bank. Burns. 2009;35:104–106
  25. Vloemans AFPM, Middelkoop E, Kreis RW. A historical appraisal of the use of cryopreserved and glycerol-preserved allograft skin in the treatment of partial thickness burns. Burns. 2002;28:S16–S20
  26. Kearney JN. Quality issues in skin banking: a review. Burns. 1998;24:299–305

PII: S0305-4179(09)00141-7

doi: 10.1016/j.burns.2009.04.020

Burns
Volume 36, Issue 3 , Pages 347-351 , May 2010

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